Abstract Helicobacter pylori is a bacterium that reaches half of the world population and it’s recognized as the main cause of chronic gastritis and peptic ulcer. In this study, we evaluated the anti-H. pylori, antioxidant and immunomodulatory activities of the methanolic (MeOH) extract of Eugenia uniflora leaves and chemical profile. Anti-H. pylori activity was evaluated by spectrophotometric broth microdilution technique by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), in addition to the evaluation of the effect on the urease enzyme. The antioxidant activity was evaluated by capturing O2 •-, HOCl e NO• radicals. The immunomodulatory effect was evaluated on the cytokines TNF-α, IL-6 and on nitric oxide through inhibition in LPS-stimulated macrophages. The chemical profile was performed by total phenolic, tannin and flavonoid contents and mass spectrometry analysis by ESI-FT-ICR MS. In the anti-H. pylori assay the extract showed MIC of 128 μg/mL, however it did not obtain MBC. The extract also showed ability to inhibit the urease enzyme about 20%. The antioxidant activity of the MeOH extract showed EC50 values of 29.77 µg/mL, 15.71 µg/mL and 442.10 µg/mL to O2 •-, HOCl and NO•, respectively. The extract also showed influence on the release of TNF-α, IL-6 and NO in LPS-stimulated macrophages, ranging from 39% to 97% inhibition. Flavonoids, phenylpropanoids, tannins, triterpenoids and carbohydrates were the major classes of compounds present in the MeOH extract as identified by (-)-ESI-FT-ICR MS. The results indicate important anti-H. pylori, antioxidant and immunomodulatory activities from Eugenia uniflora highlighting its importance in the prevention and treatment of diseases caused by H. pylori infection.

O objetivo deste artigo é relatar a experiência da parceria ensino-serviço entre os participantes do Programa de Educação pelo Trabalho para a Saúde/Redes de Atenção (PET-Redes) no município de Vitória, ES, Brasil. Discute-se a relevância das ações para incrementar a formação de profissionais para atuarem junto ao Sistema Único de Saúde (SUS). Apresentam-se as mudanças desencadeadas com a implantação dos programas, relatando a abertura para vivências multiprofissionais, destacando a experiência exitosa de utilização do Genograma e Ecomapa no processo de aproximação dos alunos com o território e usuários. Ressaltam-se desafios e fragilidades, como a dificuldade de incorporar as atividades do PET na rotina da unidade de saúde e a necessidade de mudanças da perspectiva pedagógica da formação profissional, de forma que esteja mais articulada à rede de serviços de saúde e atinja todos os alunos durante a graduação.

This paper reports the experiences of the teaching-service integration developed by the participants of the Education by Work for Health Program (PET-Health Networks) at Victoria-ES, Brazil. The effectiveness of activities to increase the education of health professionals working at the Brazilian Health System (SUS) has been discussed. Changes resulting from the program implementation are described and the openness to multiprofessional experiences are reported with particular emphasis on the success of Genogram and Ecomap in bringing students closer to the territory and users. Moreover, we discuss challenges and weaknesses, such as the difficulty in including PET activities in the healthcare unit’s routine as well as the need for a different pedagogical perspective toward professional education to ensure that students are more articulate with the healthcare services’ network and to reach out to all students prior to graduation.

El artículo relata la experiencia de asociación enseñanza-servicio entre participantes del Programa de Educación en el Trabajo para la Salud (PET-Salud/Redes). Discute la importancia de acciones para incrementar la formación de profesionales para que trabajen con el Sistema Brasileño de Salud (SUS). Presenta cambios provocados con la implantación del programa, relatando la apertura para vivencias interdisciplinares y multi profesionales, destacando la experiencia exitosa de la utilización del Genograma y Ecomapa en el proceso de aproximación de alumnos al territorio y usuarios. Resalta desafíos y fragilidades como la dificultad de incorporar las actividades del PET en la rutina de la unidad de salud y la necesidad de cambios de la perspectiva pedagógica en la formación profesional, de tal modo que esté más articulada con la red de servicios de salud y alcance de todos los alumnos durante los estudios universitarios.

O objetivo deste artigo é relatar a experiência da parceria ensino-serviço entre os participantes do Programa de Educação pelo Trabalho para a Saúde/Redes de Atenção (PET-Redes) no município de Vitória, ES, Brasil. Discute-se a relevância das ações para incrementar a formação de profissionais para atuarem junto ao Sistema Único de Saúde (SUS). Apresentam-se as mudanças desencadeadas com a implantação dos programas, relatando a abertura para vivências multiprofissionais, destacando a experiência exitosa de utilização do Genograma e Ecomapa no processo de aproximação dos alunos com o território e usuários. Ressaltam-se desafios e fragilidades, como a dificuldade de incorporar as atividades do PET na rotina da unidade de saúde e a necessidade de mudanças da perspectiva pedagógica da formação profissional, de forma que esteja mais articulada à rede de serviços de saúde e atinja todos os alunos durante a graduação.

This paper reports the experiences of the teaching-service integration developed by the participants of the Education by Work for Health Program (PET-Health Networks) at Victoria-ES, Brazil. The effectiveness of activities to increase the education of health professionals working at the Brazilian Health System (SUS) has been discussed. Changes resulting from the program implementation are described and the openness to multiprofessional experiences are reported with particular emphasis on the success of Genogram and Ecomap in bringing students closer to the territory and users. Moreover, we discuss challenges and weaknesses, such as the difficulty in including PET activities in the healthcare unit’s routine as well as the need for a different pedagogical perspective toward professional education to ensure that students are more articulate with the healthcare services’ network and to reach out to all students prior to graduation.

El artículo relata la experiencia de asociación enseñanza-servicio entre participantes del Programa de Educación en el Trabajo para la Salud (PET-Salud/Redes). Discute la importancia de acciones para incrementar la formación de profesionales para que trabajen con el Sistema Brasileño de Salud (SUS). Presenta cambios provocados con la implantación del programa, relatando la apertura para vivencias interdisciplinares y multi profesionales, destacando la experiencia exitosa de la utilización del Genograma y Ecomapa en el proceso de aproximación de alumnos al territorio y usuarios. Resalta desafíos y fragilidades como la dificultad de incorporar las actividades del PET en la rutina de la unidad de salud y la necesidad de cambios de la perspectiva pedagógica en la formación profesional, de tal modo que esté más articulada con la red de servicios de salud y alcance de todos los alumnos durante los estudios universitarios.

AbstractCoumarins are phenolic compounds and have various biological properties, including antioxidant activity. The isocoumarin paepalantine, isolated from of Paepalanthus bromelioides Silveira, Eriocaulaceae, exhibits a wide range of biological activities, including antimicrobial, anti-inflammatory, antioxidant and cytotoxic properties. Studies on paepalantine often use dimethylsulfoxide as a solvent. However the dimethylsulfoxide interferes with antimicrobial, cytotoxic and antioxidant assays. Thus, this study aims to evaluate alternative solvents for paepalantine and evaluate their potential to interfere with antioxidant assays (ABTS•+, O2•-, HOCl). Of the selected solvents, propylene glycol had good solubility and remained stable throughout the study period. The results suggested that there is no interference from propylene glycol in antioxidant assays, while dimethylsulfoxide significantly interfered with the HOCl assay. The antioxidant assays showed that paepalantine demonstrated similar or even better antioxidant activity than Trolox. Thus, propylene glycol may be the solvent of choice for paepalantine, a compound that has significant biological potential.

OBJETIVO: Analisar a composição metabólica de áreas encefálicas através da espectroscopia de prótons por ressonância magnética em crianças com transtornos do espectro alcoólico fetal e crianças normais. MÉTODO: A amostra deste estudo de casos-controles incluiu 8 meninos com história epidemiológica de exposição fetal ao álcool (idade mediana 13,6±3,8 anos), diagnosticados com transtorno do espectro alcoólico fetal, e 8 controles (idade mediana 12,1±3,4 anos). Utilizou-se voxel único de 8 cm³, tempo de eco 30 ms, tempo de repetição 1.500 ms, 128 aquisições, em scanner de 1,5T para as seguintes áreas: cíngulo anterior, lobo frontal esquerdo, estriado esquerdo e hemisfério cerebelar esquerdo. Estudaram-se os picos e as razões dos metabólitos N-acetilaspartato, colina, creatina e o mio-inositol. RESULTADOS: As crianças acometidas apresentaram diminuição da razão colina/creatina (p=0,020) no estriado esquerdo, e aumento da razão mio-inositol/creatina (p=0,048) no cerebelo esquerdo. Não houve diferença estatisticamente significativa nos valores encontrados no cíngulo anterior e lobo frontal entre os dois grupos. CONCLUSÃO: Este estudo encontrou evidências de que o estriado e o cerebelo esquerdos são acometidos pela exposição intra-uterina ao álcool. Estudos adicionais com amostras maiores são essenciais para expandir nosso conhecimento dos efeitos da exposição fetal ao álcool.

OBJECTIVE: To analyze the metabolic constitution of brain areas through proton magnetic resonance spectroscopy in children affected with fetal alcohol spectrum disorder compared with normal children. METHOD: The sample of this case-control study included eight boys with epidemiologic history of in utero exposure to alcohol (median age 13.6±3.8 years) who were diagnosed with fetal alcohol spectrum disorder, and eight controls (median age 12.1±3,4 years). An 8 cm³ single voxel approach was used, with echo time 30 ms, repetition time 1500 ms, and 128 acquisitions in a 1.5T scanner, and four brain areas were analyzed: anterior cingulate, left frontal lobe, left striatum, and left cerebellar hemisphere. Peaks and ratios of metabolites N-acetylaspartate, choline, creatine, and myo-inositol were measured. RESULTS: Children with fetal alcohol spectrum disorder showed a decrease in choline/creatine ratio (p=0.020) in left striatum and an increase in myo-inositol/creatine ratio (p=0.048) in left cerebellum compared with controls. There was no statistically significant difference in all peaks and ratios from the anterior cingulate and frontal lobe between the two groups. CONCLUSION: This study found evidence that the left striatum and left cerebellum are affected by intrauterine exposure to alcohol. Additional studies with larger samples are necessary to expand our knowledge of the effects of fetal exposure to alcohol.

Este artigo<A HREF="#nt01">1</A> busca explorar o potencial da pesquisa qualitativa, apresentando o método da história oral na sua modalidade trajetórias de vida, com o propósito de discutir a possibilidade de sua utilização em investigações científicas na profissão de Serviço Social. Situa os fundamentos epistemológicos da história oral, conferindo-lhe o caráter científico. Apresenta a modalidade trajetórias de vida como um constructo histórico e social que utiliza diferentes técnicas de entrevista para dar voz aos sujeitos até então invisíveis, anunciando as principais etapas dos procedimentos metodológicos utilizados nesta abordagem. Destaca, nas conclusões, a importância da construção desse modelo, sua projeção como uma proposta investigativa que implica um processo de compreender e analisar os universos sociais contextualizados e interconectados à luz da realidade das trajetórias de vida dos sujeitos pesquisados.

This article seeks to explore the potential of qualitative research. It presents the life trajectory modality of the oral history method, to discuss the possibility of its utilization in scientific research in the Social Work profession. The epistemological foundations of oral history are discussed to establish its scientific character. The life trajectories modality is presented as a historic and social construction that utilizes different interview techniques to give voice to previously invisible subjects, indicating the principal phases of the methodological procedures used in this approach. The conclusions highlight the importance of the construction of this model and its projection as a research proposal that implies a process of understanding and analyzing the social universes that are contextualized and interconnected, considering the realities of the life trajectories of the subjects studied.